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macs beads  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec macs beads
    Macs Beads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 835 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/macs+beads/CD4+MicroBeads%2C+human/pmc13357678-48-12-14
    Average 96 stars, based on 835 article reviews
    macs beads - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Isolation:

    Article Title: Adenosine-generating CD39 + plasmablasts predispose to successful infliximab therapy in pediatric IBD.
    Article Snippet: .. After reconstitution of the frozen samples, thawed PBMCs were immunomagnetically isolated using CD19-specific MACS beads on MS columns (Miltenyi Biotec, Milten). ..

    Article Title: Immunogenic tumor cell death and T-cell-derived IFN-γ elicit tumoricidal macrophages to potentiate OX40 immunotherapy
    Article Snippet: .. Treg cells were isolated from naive MC38 tumors using MACS beads (130-091-041, Miltenyi) and cocultured with BMDMs at a 4:1 ratio (Treg:BMDM) for 36 h. The cells were stimulated with 100 ng/mL MPLA +20 ng/mL IFNγ for 24 h. After removing non-adherent Tregs, polarized macrophages were harvested for RNA extraction and downstream qPCR analysis. ..

    Magnetic Cell Separation:

    Article Title: Adenosine-generating CD39 + plasmablasts predispose to successful infliximab therapy in pediatric IBD.
    Article Snippet: .. After reconstitution of the frozen samples, thawed PBMCs were immunomagnetically isolated using CD19-specific MACS beads on MS columns (Miltenyi Biotec, Milten). ..

    Article Title: Type I interferon responsive microglia shape cortical development and behavior
    Article Snippet: .. MACS beads (LS columns) , Miltenyi Biotec , Cat#130-042-401. .. MERSCOPE slides , Vizgen , PN#20400001.

    Article Title: ChromaFold predicts the 3D contact map from single-cell chromatin accessibility
    Article Snippet: .. Bone marrow cells were then incubated with MACS beads (CD117, Miltenyi Biotec, 130-091-224). .. Then enriched c-Kit+ cells were collected by running AutoMACS (Miltenyi Biotec) according to the manufacturer’s instructions.

    Article Title: High Fat and Sugar Diet Increases Enteric cDC1 and Oral Antigen‐Specific Tregs
    Article Snippet: Anti‐mouse Ter‐119 Biotin , eBioscience , 13‐5921‐85. .. MACS beads , Milteny Biotec , 130‐048‐102. .. LS columns , Milteny Biotec , 130‐042‐401.

    Article Title: Spatially resolved, multimodal in vivo Perturb-seq using antibody-based cell hashing
    Article Snippet: .. Lineage negative, Ckit+ cells were enriched using MACS beads (Miltenyi #130-090-858, #130-091-224). .. HPCs were then expanded for 10 days in HemEx-Type9AØ (Iwai #A5P10P01C) supplemented with 10 ng/mL SCF (PeproTech) and 100 ng/mL Tpo (PeProTech).

    Article Title: Immunogenic tumor cell death and T-cell-derived IFN-γ elicit tumoricidal macrophages to potentiate OX40 immunotherapy
    Article Snippet: .. Treg cells were isolated from naive MC38 tumors using MACS beads (130-091-041, Miltenyi) and cocultured with BMDMs at a 4:1 ratio (Treg:BMDM) for 36 h. The cells were stimulated with 100 ng/mL MPLA +20 ng/mL IFNγ for 24 h. After removing non-adherent Tregs, polarized macrophages were harvested for RNA extraction and downstream qPCR analysis. ..

    Article Title: An ICAM1-targeting chimeric costimulatory receptor mimics the immune synapse and enhances tumor-specific T cell function
    Article Snippet: PBMCs were isolated using Ficoll–Paque PLUS density gradient medium (Cytiva #17144003) and subsequently cryopreserved in cryopreservation medium composed of TexMACS medium (Miltenyi Biotec #170-076-307) and CryoStor10 (BioLife Solutions #210102) at a 1:2 ratio in liquid nitrogen tank until T cell isolation. .. After thawing, CD4 + and CD8 + T cells were enriched using MACS beads (Miltenyi Biotec # 130-045-101, #130-045-201) and cultured in TexMACS medium with 5% human AB serum (Sigma #H4522), 12.5 ng/ml IL7 (Miltenyi Biotec #170-076-111), and 12.5 ng/ml IL15 (Miltenyi Biotec #170-076-114). .. T cells were activated with CD3/CD28 Dynabeads (ThermoFisher #11141D) and transduced twice with lentivirus.

    Incubation:

    Article Title: ChromaFold predicts the 3D contact map from single-cell chromatin accessibility
    Article Snippet: .. Bone marrow cells were then incubated with MACS beads (CD117, Miltenyi Biotec, 130-091-224). .. Then enriched c-Kit+ cells were collected by running AutoMACS (Miltenyi Biotec) according to the manufacturer’s instructions.

    RNA Extraction:

    Article Title: Immunogenic tumor cell death and T-cell-derived IFN-γ elicit tumoricidal macrophages to potentiate OX40 immunotherapy
    Article Snippet: .. Treg cells were isolated from naive MC38 tumors using MACS beads (130-091-041, Miltenyi) and cocultured with BMDMs at a 4:1 ratio (Treg:BMDM) for 36 h. The cells were stimulated with 100 ng/mL MPLA +20 ng/mL IFNγ for 24 h. After removing non-adherent Tregs, polarized macrophages were harvested for RNA extraction and downstream qPCR analysis. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Immunogenic tumor cell death and T-cell-derived IFN-γ elicit tumoricidal macrophages to potentiate OX40 immunotherapy
    Article Snippet: .. Treg cells were isolated from naive MC38 tumors using MACS beads (130-091-041, Miltenyi) and cocultured with BMDMs at a 4:1 ratio (Treg:BMDM) for 36 h. The cells were stimulated with 100 ng/mL MPLA +20 ng/mL IFNγ for 24 h. After removing non-adherent Tregs, polarized macrophages were harvested for RNA extraction and downstream qPCR analysis. ..

    other:

    Article Title: Immunogenic tumor cell death and T-cell-derived IFN-γ elicit tumoricidal macrophages to potentiate OX40 immunotherapy.
    Article Snippet: The cells were cultured in RPMI medium containing 10% FBS and M-CSF (50 ng/mL, 315- 02-10UG, Peprotech) on 10 cm culture dishes.

    Cell Culture:

    Article Title: An ICAM1-targeting chimeric costimulatory receptor mimics the immune synapse and enhances tumor-specific T cell function
    Article Snippet: PBMCs were isolated using Ficoll–Paque PLUS density gradient medium (Cytiva #17144003) and subsequently cryopreserved in cryopreservation medium composed of TexMACS medium (Miltenyi Biotec #170-076-307) and CryoStor10 (BioLife Solutions #210102) at a 1:2 ratio in liquid nitrogen tank until T cell isolation. .. After thawing, CD4 + and CD8 + T cells were enriched using MACS beads (Miltenyi Biotec # 130-045-101, #130-045-201) and cultured in TexMACS medium with 5% human AB serum (Sigma #H4522), 12.5 ng/ml IL7 (Miltenyi Biotec #170-076-111), and 12.5 ng/ml IL15 (Miltenyi Biotec #170-076-114). .. T cells were activated with CD3/CD28 Dynabeads (ThermoFisher #11141D) and transduced twice with lentivirus.



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